Journal: Advanced Science

Article Title: An Unrevealed Molecular Function of Corannulene Buckybowl Glycoconjugates in Selective Tumor Annihilation by Targeting the Cancer‐Specific Warburg Effect

doi: 10.1002/advs.202105315

Figure Lengend Snippet: DNA interaction properties of Cor‐sugars. a) Fluorescence quenching of Cor‐gal in the presence of increasing amount of hsDNA. b) CD spectra of hsDNA titrated by increasing amount of Cor‐gal in Tris‐HCl buffer (pH 7.2) at different molar ratio at 25 °C. c) Thermodynamic parameters for instant binding of Cor‐gal with hsDNA by ITC study (10 × 10 −6 m Cor‐gal were titrated by 100 µM of hsDNA). d) Effects of increasing amounts of Cor‐gal on the relative viscosity of hsDNA at 25 ± 0.1 °C. e) yet another scientific artificial reality application (YASARA) Micro mediated 200 ns molecular dynamics simulation result of Cor‐gal with 15‐mer DNA (PDB: 2MG8) in a 0.9% NaCl solution at 298 K. f) Binding mode of intercalated Cor‐gal in the DNA base pairs after 200 ns MD simulation.

Article Snippet: All chemical reagents for synthesis of Cor‐sugars were obtained from commercial suppliers and used as received. pBR322 plasmid DNA and Herring sperm DNA (hsDNA) was purchased from Solarbio and stored at 2–8 °C.

Techniques: Fluorescence, Circular Dichroism, Binding Assay, Viscosity