Journal:

Article Title: DNA containing CpG motifs induces angiogenesis

doi: 10.1073/pnas.132605599

Figure Lengend Snippet: HSV DNA and CpG ODN induce angiogenesis. Pellets containing 90 ng of VEGF (A); vehicle alone (B); 2 μg of HSV DNA or control herring sperm DNA (C and D); or 1 μg of CpG or control ODN (E and F) were inserted into corneal micropockets. The degree of angiogenesis 4 days after pellet implantation is shown (40×). (G) The average degree of neovascularization induced by various amounts of HSV DNA is shown vs. 5 μg of herring sperm DNA, 90 ng of VEGF, or vehicle alone (four mice per group).

Article Snippet: Herring sperm DNA (Boehringer Mannheim) was prepared by passage through Detoxi-Gel Endotoxin Removal Gel 20344 (Pierce) to reduce endotoxin levels to <6 endotoxin units per mg. Recombinant human VEGF165 (rhVEGF), recombinant mouse VEGF (rmVEGF), mouse VEGF-neutralizing antibody, and biotinylated rat anti-mouse VEGF were purchased from R & D Systems.

Techniques:

Journal:

Article Title: DNA containing CpG motifs induces angiogenesis

doi: 10.1073/pnas.132605599

Figure Lengend Snippet: Anti-mVEGF Ab suppresses CpG DNA-induced angiogenesis. Pellets containing 45 ng of rmVEGF164, 1 μg of ODN, or 2 μg of HSV or herring sperm DNA were placed in corneal pockets. Anti-mVEGF antibody (5 μg in 5 μl of PBS) was injected subconjunctivally into the eyes just before and 2 days after pellet implantation. The eyes were observed under stereomicroscopy and neovascularization measured 4 days after pellet implantation in four mice per group. Black solid bar, +anti-mVEGF Ab.

Article Snippet: Herring sperm DNA (Boehringer Mannheim) was prepared by passage through Detoxi-Gel Endotoxin Removal Gel 20344 (Pierce) to reduce endotoxin levels to <6 endotoxin units per mg. Recombinant human VEGF165 (rhVEGF), recombinant mouse VEGF (rmVEGF), mouse VEGF-neutralizing antibody, and biotinylated rat anti-mouse VEGF were purchased from R & D Systems.

Techniques: Injection

Journal:

Article Title: DNA containing CpG motifs induces angiogenesis

doi: 10.1073/pnas.132605599

Figure Lengend Snippet: CpG DNA ODN up-regulates VEGF mRNA expression in J774A.l cells. J774A.l cells were incubated for 3–6 h with 3 μg/ml of HSV DNA, herring sperm DNA, CpG, or control ODN. Total RNA was extracted from 106 cells, reverse transcribed, and PCR amplified to detect the 120, 164, 188 isoforms of VEGF. β-actin served as the positive control and standard for this RT-PCR.

Article Snippet: Herring sperm DNA (Boehringer Mannheim) was prepared by passage through Detoxi-Gel Endotoxin Removal Gel 20344 (Pierce) to reduce endotoxin levels to <6 endotoxin units per mg. Recombinant human VEGF165 (rhVEGF), recombinant mouse VEGF (rmVEGF), mouse VEGF-neutralizing antibody, and biotinylated rat anti-mouse VEGF were purchased from R & D Systems.

Techniques: Expressing, Incubation, Amplification, Positive Control, Reverse Transcription Polymerase Chain Reaction